Anti-gtf3c3 antibody (ab168926) at 1/500 dilution + HeLa nuclear extract at 50 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique
All lanes : Anti-gtf3c3 antibody (ab168926) at 1/500 dilutionLane 1 : gtf3c3-transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique
Immunofluorescent analysis of HeLa cells, labeling gtf3c3 with purified ab168926 at 10 µg/ml. Antibody purification with Protein A will be needed prior to use.