Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue labelling GTF3C4 with ab74229 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-GTF3C4 antibody (ab74229) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgdeveloped using the ECL technique
ab74229, at 1 µg/ml (used for IP at 3 µg/mg lysate) Lane 1: HeLa whole cell lysate at 1 mg for IP (20% of IP loaded). Lane 2: control IgG. Detection: Chemiluminescence with an exposure time of 3 seconds.