All lanes : Anti-GTPBP4 antibody (ab153977) at 1/5000 dilutionLane 1 : NIH 3T3 whole cell lysateLane 2 : JC whole cell lysateLane 3 : BCL1 whole cell lysateLysates/proteins at 10 µg per lane.
All lanes : Anti-GTPBP4 antibody (ab153977) at 1/10000 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysateLane 3 : HeLa whole cell lysateLane 4 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.
Immunofluorescent analysis of A431 cells (fixed in iced-cold MeOH for 5 min) labeling GTPBP4 with ab153977 at 1/500 dilution (green). Right image shows cells co-stained with Hoechst 33342 (blue).