Anti-H Cadherin antibody
| Name | Anti-H Cadherin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab36905 |
| Prices | $394.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP IHC-P ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Human |
| Antigen | A 15 amino acid peptide from near the amino terminus of human H Cadherin |
| Description | Rabbit Polyclonal |
| Gene | CDH13 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![H Cadherin was immunoprecipitated using 0.5mg NIH3T3 whole cell extract, 5µg of Rabbit polyclonal to H Cadherin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, NIH3T3 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab36905.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 78kDa; H Cadherin.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/28607_ab36905-171838-IPV019ab369058m.jpg)
H Cadherin was immunoprecipitated using 0.5mg NIH3T3 whole cell extract, 5µg of Rabbit polyclonal to H Cadherin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, NIH3T3 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab36905.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 78kDa; H Cadherin.
Lane 1 : Anti-H Cadherin antibody (ab36905) at 0.5 µg/mlLane 2 : Anti-H Cadherin antibody (ab36905) at 1 µg/mlLane 1 : 3T3 cell lysate.Lane 2 : 3T3 cell lysate.
ab36905 at 20µg/ml staining H Cadherin in K562 cells by ICC/IF
ab36905 staining H Cadherin in human kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in paraformaldehyde and an antigen retrieval step was performed using TRIS-EDTA Buffer pH 9.0. Cells were then permeabilized and incubated with ab36905 at a 1/1000 dilution for 30 minutes at 20°C. The secondary used was an undiluted HRP conjugated goat polyclonal.See Abreview
Product References
Down-regulation of adiponectin receptors in osteoarthritic chondrocytes. - Down-regulation of adiponectin receptors in osteoarthritic chondrocytes.
Wang Q, Cai J, Wang J, Xiong C, Yan L, Zhang Z, Fang Y, Zhao J. Cell Biochem Biophys. 2014 Sep;70(1):491-7.
H-cadherin expression reduces invasion of malignant melanoma. - H-cadherin expression reduces invasion of malignant melanoma.
Kuphal S, Martyn AC, Pedley J, Crowther LM, Bonazzi VF, Parsons PG, Bosserhoff AK, Hayward NK, Boyle GM. Pigment Cell Melanoma Res. 2009 Jun;22(3):296-306. doi: