All lanes : Anti-H6PD antibody [2A7] (ab119046) at 1/250 dilutionLane 1 : HEK293T lysate transfected with pCMV6-ENTRY control cDNA Lane 2 : HEK293T lysate transfected with pCMV6-ENTRY H6PD cDNA Lysates/proteins at 5 µg per lane.
All lanes : Anti-H6PD antibody [2A7] (ab119046) at 1/250 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : HT29 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.
ab119046, at 1/50 dilution, staining H6PD in Paraffin-embedded Human colon by Immunohistochemistry.
ab119046, at 1/50 dilution, staining H6PD in Paraffin-embedded Human liver by Immunohistochemistry.
ab119046, at 1/50 dilution, staining H6PD in Paraffin-embedded Human pancreas by Immunohistochemistry.
ab119046, at 1/50 dilution, staining H6PD in Paraffin-embedded Human prostate by Immunohistochemistry.
ab119046 at 1/100 dilution staining H6PD in COS7 cells transiently transfected with pCMV6-ENTRY H6PD by Immunofluorescence.
ab119046 at 1/100 dilution, staining H6PD in HEK293T cells transfected with pCMV6-ENTRY H6PD overexpress plasmid(Red) or empty vector control plasmid(Blue) were by flow cytometry.
ab119046 at 1/100 dilution staining H6PD in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).