Anti-HADHA antibody [2C2AA5]

• Supplier: Abcam
• Catalog: ab110281
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone: 2C2AA5
• Applications: WB ICC/IF ICC/IF FC ELISA
• Species Reactivities: Human
• Antigen: Human heart mitochondria (UniProt ID: P40939)
• Description: Mouse Monoclonal
• Gene: HADHA
• Conjugate: Unconjugated

Product images

All lanes : Anti-HADHA antibody [2C2AA5] (ab110281) at 0.5 µg/mlLane 1 : Human heart mitochondria Lane 2 : Human liver mitochondria Lane 3 : HepG2 mitochondria Lysates/proteins at 5 µg per lane.

Immunocytochemistry image of ab110281 stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with the antibody (ab110281, 4 µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (green) AlexaFluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1 h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.

HeLa cells were stained with 1 µg/mL ab110281 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.