All lanes : Anti-HADHA antibody - C-terminal (ab135791) at 1/100 dilutionLane 1 : A2058 cell line lysateLane 2 : A375 cell line lysateLane 3 : NCI-H460 cell line lysateLane 4 : Ramos cell line lysateLysates/proteins at 35 µg per lane.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma tissue labelling HADHA with ab135791 at 1/50 dilution, followed by peroxidase-conjugated secondary antibody and DAB staining.
Flow cytometry analysis of Ramos cells labelling HADHA with ab135791 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.