Anti-HADHSC antibody (ab154088) at 1/1000 dilution + mouse liver whole cell lysate at 50 µg
All lanes : Anti-HADHSC antibody (ab154088) at 1/1000 dilutionLane 1 : 293T whole cell lysate/extractLane 2 : A431 whole cell lysate/extractLane 3 : HeLa whole cell lysate/extractLane 4 : HepG2 whole cell lysate/extractLysates/proteins at 30 µg per lane.
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling HADHSC with ab154088 at 1/250 dilution.
Immunofluorescence analysis of HeLa cells (fixed with 2% paraformaldehyde/culture medium at 37 °C for 30 min) labeling HADHSC with ab154088 at 1/500 dilution (green). The image in the right panel is costained with Hoechst 33342 (blue).