IHC image of hair cortex Cytokeratin staining in Human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112444, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
All lanes : Anti-hair cortex Cytokeratin antibody (ab112444) at 1 µg/mlLane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell LysateLane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell LysateLane 4 : LOVO (Human colon adenocarcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.