Anti-HARS antibody [HARSA6]

• Supplier: Abcam
• Catalog: ab50835
• Prices: $370.00
• Sizes: 50 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: HARSA6
• Applications: IHC-P ICC/IF IP FC WB
• Species Reactivities: Human
• Antigen: HARS recombinant fragment (Human), containing 50-200 AAs from near the C-terminal
• Description: Mouse Monoclonal
• Gene: HARS
• Conjugate: Unconjugated

Product images

Anti-HARS antibody [HARSA6] (ab50835) at 1/100 dilution + Hela whole cell lysate at 25 µgSecondaryMouse IgG antibody at 1/2500 dilution

ab50835 at a 1/10 dilution staining HARS in Hela cells, using Alexa Fluor®488 Goat Anti-mouse IgG at 1/200 dilution.

ab50835 at a 1/10 dilution staining HARS using Alexa Fluor® 488 Goat Anti-mouse IgG at 1/400 dilution.

Anti-HARS antibody [HARSA6] (ab50835) at 1/100 dilution + F2408 whole cell lysate at 25 µgSecondaryMouse IgG antibody at 1/2500 dilution

IHC image of ab50835 staining in human cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50835, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

Mechanical-stretch of C2C12 myoblasts inhibits expression of Toll-like receptor 3 - Mechanical-stretch of C2C12 myoblasts inhibits expression of Toll-like receptor 3

Chen R, Feng L, Ruan M, Liu X, Adriouch S, Liao H. PLoS One. 2013 Nov 4;8(11):e79930.