Anti-HB EGF antibody
| Name | Anti-HB EGF antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab92620 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ELISA |
| Species Reactivities | Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human HB EGF |
| Description | Rabbit Polyclonal |
| Gene | HBEGF |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab92620 stained MG63 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab92620 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Anti-HB EGF antibody (ab92620) at 1 µg/ml + Stomach (Human) Whole Cell Lysate - tumor tissue (ab29678) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.