These images shows Vero cells carrying human HG-EGF expression vector (left hand image) and Vero cells carrying mouse HB-EGF expression vector (right hand image). Cells treated with ab66793, fixed with 4% PFA and reacted with Cys3 conjugated 2nd antibody.
All lanes : HB EGF antibody [4G10](biotin) - Azide free (ab66793) at 1 µg/ml. Lane 1 : Vero cell extract Lane 2 : Vero cell extract from cells carrrying human HB-EGF expression vector. Lane 3 : Vero cell extract from cells carrying mouse HB-EGF expression vector
All lanes : Anti-HB EGF antibody [4G10] (Biotin) (ab66793) at 1 µg/mlLane 1 : Vero cell extractLane 2 : Vero cell extract from cells carrrying human HB-EGF expression vector. Lane 3 : Vero cell extract from cells carrying mouse HB-EGF expression vector.