![Western blot analysis on immunoprecipitation from 1) K562 cell lysate and 2) PBS, labeling HB EGF using ab185555 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/29024_ab185555-220183-ab185555IP.jpg)
Western blot analysis on immunoprecipitation from 1) K562 cell lysate and 2) PBS, labeling HB EGF using ab185555 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.
![All lanes : Anti-HB EGF antibody [EPR13276(2)] (ab185555) at 1/20000 dilutionLane 1 : HeLa cell lysateLane 2 : K562 cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/29025_ab185555-220182-ab185555WB.jpg)
All lanes : Anti-HB EGF antibody [EPR13276(2)] (ab185555) at 1/20000 dilutionLane 1 : HeLa cell lysateLane 2 : K562 cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique