All lanes : Anti-HCN3 [EPR13494(2)] antibody (ab192025) at 1/10000 dilutionLane 1 : Human cerebellum lysateLane 2 : Human fetal brain lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-HCN3 [EPR13494(2)] antibody (ab192025) at 1/1000 dilutionLane 1 : Human forebrain lysateLane 2 : Human hypothalamus lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Western blot analysis of HCN3 in Human fetal brain lysate immunoprecipitated using ab192025 at 1/30 dilution.Secondary antibody: Goat anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/1000 dilution. Blocking/dilution buffer: 5% NFDM/TBST.