Anti-HDAC10 antibody
| Name | Anti-HDAC10 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab53096 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P ELISA IHC-F |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide (Human) |
| Description | Rabbit Polyclonal |
| Gene | HDAC10 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab53096, at 1/50 dilution, staining HDAC10 in paraffin embedded human lung carcinoma tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.
All lanes : Anti-HDAC10 antibody (ab53096) at 1/300 dilutionLane 1 : Cos7 cell extractLane 2 : Cos7 cell extract with peptide
ICC/IF image of ab53096 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53096, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Monoaminergic and neuropeptidergic neurons have distinct expression profiles of - Monoaminergic and neuropeptidergic neurons have distinct expression profiles of
Takase K, Oda S, Kuroda M, Funato H. PLoS One. 2013;8(3):e58473.
Fasting and high-fat diet alter histone deacetylase expression in the medial - Fasting and high-fat diet alter histone deacetylase expression in the medial
Funato H, Oda S, Yokofujita J, Igarashi H, Kuroda M. PLoS One. 2011 Apr 15;6(4):e18950.