Anti-HDAC2 antibody
| Name | Anti-HDAC2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab16032 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F IHC-P ICC/IF ICC/IF WB IP |
| Species Reactivities | Mouse, Rat, Human, Monkey |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human HDAC2 |
| Blocking Peptide | Human HDAC2 peptide |
| Description | Rabbit Polyclonal |
| Gene | HDAC2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-HDAC2 antibody (ab16032) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![HDAC2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5ug of Rabbit polyclonal to HDAC2 and 50µl of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10min under agitation. No antibody was added to the control (lane 2). Hela whole cell extractdiluted in RIPA buffer was added to each sample and incubated for 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16032. Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697). Band: 60ka: HDAC2.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/29339_HDAC2-Primary-antibodies-ab16032-14.jpg)
HDAC2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5ug of Rabbit polyclonal to HDAC2 and 50µl of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10min under agitation. No antibody was added to the control (lane 2). Hela whole cell extractdiluted in RIPA buffer was added to each sample and incubated for 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16032. Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697). Band: 60ka: HDAC2.
ICC/IF image of ab16032 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab16032, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 phalloidin was used to label F-actin (red).
ab16032 (1/250) staining HDAC2 in paraffin-embedded Human tonsil tissue. Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval. The secondary antibody was goat anti rabbit/mouse conjugated to HRP. For further experimental details please refer to abreview.See Abreview
IHC-Fr image of HDAC2 (ab16032) staining on Rat spinal cord. The sections required antigen retrieval with sodium citrate buffer was necessary to obtain full signal strength (sodium citrate 10mM, pH6)See Abreview
All lanes : Anti-HDAC2 antibody (ab16032) at 0.4 µg/mlLane 1 : Mouse 3T3 lysateLane 2 : Rat liver lysateLane 3 : Chicken liver lysateLysates/proteins at 20 µg/ml per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
Regional and cell-type specific distribution of HDAC2 in the adult mouse brain. - Regional and cell-type specific distribution of HDAC2 in the adult mouse brain.
Yao ZG, Zhang L, Huang L, Zhu H, Liu Y, Ma CM, Sheng SL, Qin C. Brain Struct Funct. 2013 Mar;218(2):563-73.
An N-terminal truncated carboxypeptidase E splice isoform induces tumor growth - An N-terminal truncated carboxypeptidase E splice isoform induces tumor growth
Lee TK, Murthy SR, Cawley NX, Dhanvantari S, Hewitt SM, Lou H, Lau T, Ma S, Huynh T, Wesley RA, Ng IO, Pacak K, Poon RT, Loh YP. J Clin Invest. 2011 Mar;121(3):880-92.
A role for the histone deacetylase HDAC4 in the life-cycle of HIV-1-based - A role for the histone deacetylase HDAC4 in the life-cycle of HIV-1-based
Smith JA, Yeung J, Kao GD, Daniel R. Virol J. 2010 Sep 16;7:237.
Heat-shock factor 1 controls genome-wide acetylation in heat-shocked cells. - Heat-shock factor 1 controls genome-wide acetylation in heat-shocked cells.
Fritah S, Col E, Boyault C, Govin J, Sadoul K, Chiocca S, Christians E, Khochbin S, Jolly C, Vourc'h C. Mol Biol Cell. 2009 Dec;20(23):4976-84.
Identification of cellular proteins that maintain retroviral epigenetic - Identification of cellular proteins that maintain retroviral epigenetic
Poleshko A, Palagin I, Zhang R, Boimel P, Castagna C, Adams PD, Skalka AM, Katz RA. J Virol. 2008 Mar;82(5):2313-23. Epub 2007 Dec 19.
UV radiation regulates Mi-2 through protein translation and stability. - UV radiation regulates Mi-2 through protein translation and stability.
Burd CJ, Kinyamu HK, Miller FW, Archer TK. J Biol Chem. 2008 Dec 12;283(50):34976-82.
SYT-SSX1 and SYT-SSX2 interfere with repression of E-cadherin by snail and slug: - SYT-SSX1 and SYT-SSX2 interfere with repression of E-cadherin by snail and slug:
Saito T, Nagai M, Ladanyi M. Cancer Res. 2006 Jul 15;66(14):6919-27.