Anti-HDAC2 antibody [Y461]

• Supplier: Abcam
• Catalog: ab32117
• Prices: $404.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: Y461
• Applications: WB IHC-P ICC/IF ICC/IF FC IP IHC-F
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to residues in the C terminal of human HDAC2
• Description: Rabbit Monoclonal
• Gene: HDAC2
• Conjugate: Unconjugated

Product images

Overlay histogram showing HeLa cells stained with ab32117 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32117, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

All lanes : Anti-HDAC2 antibody [Y461] (ab32117) at 1/1000 dilutionLane 1 : Rat brain tissue homogenate at 20 µgLane 2 : Rat brain tissue homogenate at 40 µgLane 3 : Rat brain tissue homogenate, P1 nuclear fraction at 20 µgLane 4 : Rat brain tissue homogenate, P1 nuclear fraction at 40 µgLane 5 : Rat brain tissue homogenate, P2 non-nuclear fraction at 20 µgLane 6 : Rat brain tissue homogenate, P2 non-nuclear fraction at 40 µgSecondaryHRP-conjugated goat anti-rabbit IgG polyclonal at 1/1000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-HDAC2 antibody [Y461] (ab32117) at 1/2000 dilution + K562 cell lysate

Immunohistochemical analysis of HDAC2 expression in paraffin embedded human breast carcinoma tissue section, using 1/250 ab32117.

Immunofluorescent analysis of HDAC expression in HeLa cells, using 1/250 ab32117.

ab32117 staining HDAC2 in Rat spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/500 in PBS + 0.2% TritonX + 1% BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Antigen unmasking with sodium citrate buffer (10mM sodium citrate, 0.05% Tween 20, ph6) was necessary to obtain a good signal. The sections were counterstained with DAPI.See Abreview

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