Anti-HDAC3 antibody

• Supplier: Abcam
• Catalog: ab16047
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P ICC/IF ICC/IF ICC/IF WB IP
• Species Reactivities: Mouse, Rat, Human, Deer, Zebrafish, Chicken, Xenopus
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human HDAC3
• Blocking Peptide: Human HDAC3 peptide
• Description: Rabbit Polyclonal
• Gene: HDAC3
• Conjugate: Unconjugated

Product images

Lanes 1 & 6 : Anti-HDAC3 antibody (ab16047) at 1 µg/ml (Sample: HeLa nuclear lysate, 20 ug)Lanes 2 & 7 : Anti-HDAC3 antibody (ab16047) at 1 µg/ml (Sample: HeLa whole cell lysate, 20 ug)Lanes 3 & 8 : Anti-HDAC3 antibody (ab16047) at 1 µg/ml (Sample: A431 cell lysate, 20 ug)Lanes 4 & 9 : Anti-HDAC3 antibody (ab16047) at 1 µg/ml (Sample: Jurkat cell lysate, 20 ug)Lanes 5 & 10 : Anti-HDAC3 antibody (ab16047) at 1 µg/ml (Sample: HEK293 cell lysate, 20 ug)Lane 1 : As aboveLane 2 : As aboveLane 3 : As aboveLane 4 : As aboveLane 5 : As aboveLane 6 : Human HDAC3 peptide (ab16279) at 1 µg/mlLane 7 : Human HDAC3 peptide (ab16279) at 1 µg/mlLane 8 : Human HDAC3 peptide (ab16279) at 1 µg/mlLane 9 : Human HDAC3 peptide (ab16279) at 1 µg/mlLane 10 : Human HDAC3 peptide (ab16279) at 1 µg/mlSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilutionPerformed under reducing conditions.

SK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.5% Triton X-100 and incubated for 1 hour with ab16047 (1/100). The antibody clearly stained the nucleus (red). The cells were counterstained with DAPI (blue). 100x magnification.

Interphase HeLa cells incubated with ab16047 (1/500). The antibody shows predominantly nuclear staining. The ab16047 staining is shown in green. The cells were counterstained with DAPI (red). 

ab16047 staining HDAC3 in human breast cancer tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/2000 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.See Abreview

All lanes : Anti-HDAC3 antibody (ab16047) at 1 µg/mlLane 1 : Mouse 3T3 cell lysateLane 2 : Rat liver cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

HDAC3 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to HDAC3 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16047.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 49kDa: HDAC3.

All lanes : Anti-HDAC3 antibody (ab16047) at 1 µg/mlLane 1 : MarkerLane 2 : Zebrafish brain homogenate (20ug)Lane 3 : Zebrafish heart homogenate (20ug)Lane 4 : Zebrafish liver homogenate (20ug)SecondaryDonkey polyclonal to Goat IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Product References

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Deletion of histone deacetylase 3 reveals critical roles in S phase progression - Deletion of histone deacetylase 3 reveals critical roles in S phase progression

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