Western blot analysis of extracts from Raji and MCF7 cells, untreated (-) or treated with TPA #4174 (200 nM, 30 min; +), using Phospho-Bim (Ser77) (D4H12) Rabbit mAb (upper) or Bim (C34C5) Rabbit mAb #2933 (lower).
Immunoprecipitation of Phospho-Bim (Ser77) from extracts of MCF7 cells treated with TPA #4174 (200 nM, 30 min), using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Phospho-Bim (Ser77) (D4H12) Rabbit mAb (lane 3). Lane 1 is 10% input. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used to avoid recognition of rabbit IgG heavy and light chains by western blot.