Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli, nuclear membrane, cytoplasm and golgi apparatus. Recommended concentration of ab122131 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-HEATR6 antibody (ab122131) at 1/250 dilutionLane 1 : RT-4 cell lysateLane 2 : U-251 MG cell lysateLane 3 : Human PlasmaLane 4 : Human Liver lysateLane 5 : Human Tonsil lysatedeveloped using the ECL technique
ab122131, at a 1/200 dilution, staining HEATR6 in paraffin embedded Human kidney tissue by Immunohistochemistry.