Anti-Hepcidin-25 antibody

• Supplier: Abcam
• Catalog: ab30760
• Prices: $400.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB IHC-F IHC-P ELISA
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Hepcidin-25
• Description: Rabbit Polyclonal
• Gene: HAMP
• Conjugate: Unconjugated

Product images

Anti-Hepcidin-25 antibody (ab30760) + LPS treated mouse serumSecondaryDonkey Polyclonal to Anti Rabbit IgGdeveloped using the ECL techniquePerformed under reducing conditions.

Hepcidin-25 expression in Rat liver cells using Rabbit polyclonal to Hepcidin-25 (ab30760).Immunohistochemistry was performed on PFA fixed cells, ab30760 was used at 1/1000 incubated overnight at RT. Haemotoxylin is used as a nuclear counterstain in this image.

Anti-Hepcidin-25 antibody (ab30760) at 1 µg/ml + HAMP Human Full-length Recombinant Protein (Tagged) at 0.1 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

ab30760 at a 1/200 dilution staining Hepcidin-25 in human artery tissue sections by Immunohistochemistry (frozen sections) incubated for 24 hours at 20°C. Acetone fixed. Blocked with 0.5% BSA for 10 minutes at 20°C. Secondary used at 7µg/ml Goat anti-rabbit IgG (H+L) conjugated to biotin.Immunoperoxydase system and AEC were used to develop the color.See Abreview

ab30760 staining cultured rat astrocytes by ICC/IF. The cultured cells were fixed with 4% paraformaldehyde for 5 minutes and blocked with 10% donkey serum in 0.1% PBS-0.3% TritonX for 30 minutes at 24°C. The cultured cells were then stained with ab30760 at 1/100 in 0.3% TritonX with 0.1% PBS and 10% donkey serum for 24h at 4°C. An Alexa Fluro 488 donkey anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei were stained with 1.43µM Hoechst and can be observed in blue. Hepcidin expression visible perinuclearly,  mainly in the lysosomes.See Abreview

Product References

Hepcidin expression in colon during trinitrobenzene sulfonic acid-induced colitis - Hepcidin expression in colon during trinitrobenzene sulfonic acid-induced colitis

Gotardo EM, Ribeiro Gde A, Clemente TR, Moscato CH, Tome RB, Rocha T, Pedrazzoli J Jr, Ribeiro ML, Gambero A. World J Gastroenterol. 2014 Apr 21;20(15):4345-52.

Aberrant gene expression in dogs with portosystemic shunts. - Aberrant gene expression in dogs with portosystemic shunts.

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The systemic iron-regulatory proteins hepcidin and ferroportin are reduced in the - The systemic iron-regulatory proteins hepcidin and ferroportin are reduced in the

Raha AA, Vaishnav RA, Friedland RP, Bomford A, Raha-Chowdhury R. Acta Neuropathol Commun. 2013 Sep 3;1(1):55.

Pretreatment with CO-releasing molecules suppresses hepcidin expression during - Pretreatment with CO-releasing molecules suppresses hepcidin expression during

Shin DY, Chung J, Joe Y, Pae HO, Chang KC, Cho GJ, Ryter SW, Chung HT. Blood. 2012 Mar 15;119(11):2523-32.

Knockdown of beta2-microglobulin perturbs the subcellular distribution of HFE and - Knockdown of beta2-microglobulin perturbs the subcellular distribution of HFE and

Bhatt L, Horgan CP, McCaffrey MW. Biochem Biophys Res Commun. 2009 Jan 23;378(4):727-31. doi: