Anti-HERV-FRD antibody
| Name | Anti-HERV-FRD antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab90733 |
| Prices | $370.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Human, Chimpanzee |
| Antigen | A synthetic peptide corresponding to a region within N terminal amino acids 36 - 85 ( GSPYSTNCWLCTSSSTETPGTAYPASPREWTSIEAELHISYRWDPNLKGL ) of Human HERV-FRD (NP_997465) Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | ERV3-1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab90733 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab90733 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-HERV-FRD antibody (ab90733) at 1 µg/ml (in 5% skim milk / PBS buffer) + HT1080 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution