All lanes : Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] (ab181337) at 1/10000 dilutionLane 1 : Jurkat cell lysateLane 2 : K562 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin embedded Human transitional cell carcinoma of bladder tissue sections labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) using ab181337 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Immunofluorescent analysis of paraformaldehyde fixed A431 cells labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) using ab181337 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) was used as the secondary at a 1/200 dilution.
Flow Cytometry analysis of K562 cells labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) using ab181337 at a 1/20 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.