All lanes : Anti-Hexokinase II antibody [3D3] (ab104836) at 1/500 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : HEK293 cell lysate
ab104836 at 1/200 dilution staining Hexokinase II in Human esophagus cancer by Immunohistochemistry, Paraffin-embedded tissue with DAB staining.
ab104836 at 1/200 dilution staining Hexokinase II in Human lung cancer by Immunohistochemistry, Paraffin-embedded tissue with DAB staining.
ab104836 staining Hexokinase II in K562 cells by Immunofluorescence (green) and negative control (purple).
Immunohistochemical analysis of Human skin tissue, staining Hexokinase II with ab104836.Tissue was fixed with paraformaldehyde and blocked with 10% BSA for 2 hours at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/250 in 5% NGS + 10% BSA in PBS) for 16 hours at 4°C. An HRP-conjugated goat anti-mouse polyclonal IgG (1/250) was used as the secondary antibody. See Abreview