Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human transitional cell carcinoma of bladder sections labeling HGFA Inhibitor 1 with ab189511 at a 1/100 dilution. Secondary antibody used was a ready to use HRP polymer for rabbit IgG. Counterstained with hematoxylin.
Anti-HGFA Inhibitor 1 antibody [EPR15652] (ab189511) at 1/10000 dilution + HeLa cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Anti-HGFA Inhibitor 1 antibody [EPR15652] (ab189511) at 1/50000 dilution + HCT116 cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Anti-HGFA Inhibitor 1 antibody [EPR15652] (ab189511) at 1/50000 dilution + Human placenta lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution