![Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling HIF-1-alpha with ab179483 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic and weak nuclear staining on HeLa cell line. The expression of HIF-1-alpha was increased upon CoCl2 treatment (0.6nM for 6 hours); and the protein also translocated from the cytoplasm into the nucleus. [Biochimica et Biophysica Acta 1745 (2005) 187 – 195.] The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:1. ab179483 at 1/50 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dil](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/562_ab179483-244389-ab179483.jpg)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling HIF-1-alpha with ab179483 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic and weak nuclear staining on HeLa cell line. The expression of HIF-1-alpha was increased upon CoCl2 treatment (0.6nM for 6 hours); and the protein also translocated from the cytoplasm into the nucleus. [Biochimica et Biophysica Acta 1745 (2005) 187 – 195.] The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:1. ab179483 at 1/50 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dil
![All lanes : Anti-HIF-1-alpha antibody [EPR16897] (ab179483) at 1/1000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate treated with 0.5mM CoCl2 (Cobalt(II) chloride) for 6 hours.Lane 2 : Untreated HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/563_ab179483-237957-ab179483-wb-1.jpg)
All lanes : Anti-HIF-1-alpha antibody [EPR16897] (ab179483) at 1/1000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate treated with 0.5mM CoCl2 (Cobalt(II) chloride) for 6 hours.Lane 2 : Untreated HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
![All lanes : Anti-HIF-1-alpha antibody [EPR16897] (ab179483) at 1/1000 dilutionLane 1 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysate treated with 0.1mM CoCl2 for 24 hours.Lane 2 : untreated NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/564_ab179483-237956-ab179483-wb-2.jpg)
All lanes : Anti-HIF-1-alpha antibody [EPR16897] (ab179483) at 1/1000 dilutionLane 1 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysate treated with 0.1mM CoCl2 for 24 hours.Lane 2 : untreated NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution