All lanes : Anti-HIPK4 antibody (ab69565) at 1/500 dilutionLane 1 : extracts from HUVEC cellsLane 2 : extracts from HUVEC cells with immunizing peptide at 10 µgLysates/proteins at 30 µg per lane.
Immunohistochemistry analysis of paraffin-embedded human testis tissue using 1:50 ab69565. For the right hand image the antibody was pre-incubated with the immunizing peptide.
Immunofluorescence analysis of A549 cells, using 1:500 ab69565. For the right hand image, the antibody was pre-incubated with the immunizing peptide.