All lanes : Anti-Histone H1 (tri methyl K25) antibody (ab17347) at 1 µg/mlLane 1 : HeLa nuclear lysateLane 2 : HeLa whole cell lysateLane 3 : HeLa nuclear lysate with Human Histone H1 (tri methyl K25) peptide (ab17587) at 1 µg/mlLane 4 : HeLa whole cell lysate with Human Histone H1 (tri methyl K25) peptide (ab17587) at 1 µg/mlLane 5 : HeLa nuclear lysate with Human Histone H1 peptide (ab17588) at 1 µg/mlLane 6 : HeLa whole cell lysate with Human Histone H1 peptide (ab17588) at 1 µg/mlLane 7 : HeLa nuclear lysate with Human Histone H1 (di methyl K25) peptide (ab21998) at 1 µg/mlLane 8 : HeLa whole cell lysate with Human Histone H1 (di methyl K25) peptide (ab21998) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution at 1/10000 dilution
Image courtesy of Human Protein Atlas ab17347 staining histone H1 tri methyl K25 in female cerebellum, showing a distinct and strong staining pattern at cells in the granular and molecular layers. Paraffin embedded human skin tissue was incubated with ab17347(1/300 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab17347 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org.
ICC/IF image of ab17347 stained human HepG2 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab17347, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293, MCF7 cells.