All lanes : Anti-Histone H1.0 antibody (ab154111) at 1/5000 dilutionLane 1 : HepG2 whole cell lysateLane 2 : HepG2 nuclear lysateLysates/proteins at 30 µg per lane.
Immunofluorescent analysis of paraformaldehyde fixed U20S cells labeling Histone H1 with ab154111 (green) at 1/500 dilution. Alpha tubulin filaments were labeled with an Alpha tubulin antibody (red) at 1/2000.
Immunohistochemical analysis of paraffin embedded SkHep1 xenograft tissue labeling Histone H1 with ab154111 at 1/500 dilution.
Immunohistochemical analysis of paraffin embedded C2C12 xenograft tissue labeling Histone H1 with ab154111 at 1/500 dilution.