Anti-Histone H1.0 antibody [27]
| Name | Anti-Histone H1.0 antibody [27] |
|---|---|
| Supplier | Abcam |
| Catalog | ab11080 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 27 |
| Applications | ICC/IF ICC/IF WB EMSA IHC-F FC IHC-P |
| Species Reactivities | Mouse, Rat, Bovine, Human, Xenopus, Vertebrates |
| Antigen | Ox Liver Histone H1 |
| Description | Mouse Monoclonal |
| Gene | H1F0 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![IHC image of Histone H1 staining in a section of formalin-fixed paraffin-embedded [human normal colon]*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was then incubated with ab11080, 1/1000 dilution, for 15 mins at room temperature. A goat anti-mouse biotinylated secondary antibody (ab6788, 1/1000 dilution), was used to detect the primary, and visualized using an HRP conjugated ABC system. Streptavidin HRP was used, ab7403 at a 1/10000 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/913_ab11080-242968-ab11080-1-01ugml-ab6788-2-1ugml-hu-colon-x40.jpg)
IHC image of Histone H1 staining in a section of formalin-fixed paraffin-embedded [human normal colon]*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was then incubated with ab11080, 1/1000 dilution, for 15 mins at room temperature. A goat anti-mouse biotinylated secondary antibody (ab6788, 1/1000 dilution), was used to detect the primary, and visualized using an HRP conjugated ABC system. Streptavidin HRP was used, ab7403 at a 1/10000 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody
IHC image of Histone H1.0 staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab11080, 7.5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97240, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
IHC image of Histone H1.0 staining in Human pancreas adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11080, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![All lanes : Anti-Histone H1.0 antibody [27] (ab11080) at 1/500 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear LysateLane 3 : Histone H1.0 Human Recombinant Protein Lysates/proteins at 30 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/916_Histone-H10-Primary-antibodies-ab11080-5.jpg)
All lanes : Anti-Histone H1.0 antibody [27] (ab11080) at 1/500 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear LysateLane 3 : Histone H1.0 Human Recombinant Protein Lysates/proteins at 30 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab11080 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11080, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing HeLa cells stained with ab11080 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11080, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/918_Histone-H10-Primary-antibodies-ab11080-7.jpg)
Overlay histogram showing HeLa cells stained with ab11080 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11080, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Mouse oocytes and early embryos express multiple histone H1 subtypes. - Mouse oocytes and early embryos express multiple histone H1 subtypes.
Fu G, Ghadam P, Sirotkin A, Khochbin S, Skoultchi AI, Clarke HJ. Biol Reprod. 2003 May;68(5):1569-76. Epub 2002 Dec 11.
Histone H1 diversity: bridging regulatory signals to linker histone function. - Histone H1 diversity: bridging regulatory signals to linker histone function.
Khochbin S. Gene. 2001 Jun 13;271(1):1-12.
Somatic linker histone H1 is present throughout mouse embryogenesis and is not - Somatic linker histone H1 is present throughout mouse embryogenesis and is not
Adenot PG, Campion E, Legouy E, Allis CD, Dimitrov S, Renard J, Thompson EM. J Cell Sci. 2000 Aug;113 ( Pt 16):2897-907.
In situ analysis of chromatin proteins during development and cell - In situ analysis of chromatin proteins during development and cell
Grunwald D, Gorka C, Curtet S, Khochbin S. Methods Mol Biol. 1999;119:443-54.
Differential recognition of histone H10 by monoclonal antibodies during cell - Differential recognition of histone H10 by monoclonal antibodies during cell
Gorka C, Brocard MP, Curtet S, Khochbin S. J Biol Chem. 1998 Jan 9;273(2):1208-15.
Variation of H1(0) content throughout the cell cycle in regenerating rat liver. - Variation of H1(0) content throughout the cell cycle in regenerating rat liver.
Gorka C, Lawrence JJ, Khochbin S. Exp Cell Res. 1995 Apr;217(2):528-33.
Accumulation of histone H1(0) during early Xenopus laevis development. - Accumulation of histone H1(0) during early Xenopus laevis development.
Grunwald D, Lawrence JJ, Khochbin S. Exp Cell Res. 1995 Jun;218(2):586-95.
Developmentally regulated chromatin acetylation and histone H1(0) accumulation. - Developmentally regulated chromatin acetylation and histone H1(0) accumulation.
Seigneurin D, Grunwald D, Lawrence JJ, Khochbin S. Int J Dev Biol. 1995 Aug;39(4):597-603.