Anti-Histone H1.2 antibody
| Name | Anti-Histone H1.2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab17677 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P IP WB |
| Species Reactivities | Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H1 |
| Blocking Peptide | Human Histone H1.2 peptide |
| Description | Rabbit Polyclonal |
| Gene | HIST1H1C |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Histone H1.2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Histone H1.2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab17677.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 35kDa; Histone H1.2](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/939_ab17677-196684-IPV022ab1767720mMod.jpg)
Histone H1.2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Histone H1.2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab17677.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 35kDa; Histone H1.2
All lanes : Anti-Histone H1.2 antibody (ab17677) at 1/1000 dilutionLane 1 : Recombinant histone H1Lane 2 : Recombinant histone H1.1Lane 3 : Recombinant histone H1.2Lane 4 : Recombinant histone H1.3Lane 5 : Recombinant histone H1.4Lane 6 : Recombinant histone H1.5
Image courtesy of Human Protein Atlas ab17677 staining histone H1.2 in human testis, showing a distinct and strong staining pattern in ductus seminiferus and leydig cells. Paraffin embedded human skin tissue was incubated with ab17677 (1/1200 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab17677 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org.
ICC/IF image of ab17677 stained human MCF7 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab17677, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and HepG2 cells.
All lanes : Anti-Histone H1.2 antibody (ab17677) at 1 µg/mlLane 1 : Histone H1 Recombinant ProteinLane 2 : Histone H1.2 (Human) - Recombinant Protein Lane 3 : Histone H1 Recombinant Protein with Human Histone H1.2 peptide (ab18500) at 1 µg/mlLane 4 : Histone H1.2 (Human) - Recombinant Protein with Human Histone H1.2 peptide (ab18500) at 1 µg/mlLysates/proteins at 0.1 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
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Tinnikov AA, Samuels HH. PLoS One. 2013 Apr 15;8(4):e61085.
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Uras IZ, List T, Nijman SM. PLoS One. 2012;7(2):e31003.
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Bronze-da-Rocha E, Lin CM, Shimura T, Aladjem MI. Int J Biol Sci. 2011 Feb 17;7(2):193-208.
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Terme JM, Sese B, Millan-Arino L, Mayor R, Izpisua Belmonte JC, Barrero MJ, Jordan A. J Biol Chem. 2011 Oct 14;286(41):35347-57.
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Johann DJ, Rodriguez-Canales J, Mukherjee S, Prieto DA, Hanson JC, Emmert-Buck M, Blonder J. J Proteome Res. 2009 May;8(5):2310-8.
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Sancho M, Diani E, Beato M, Jordan A. PLoS Genet. 2008 Oct;4(10):e1000227.