All lanes : Anti-Histone H1.2 antibody [EPR12691] (ab181977) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : 293 cell lysateLane 3 : A375 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling Histone H1.2 with ab181977 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Immunofluorescent analysis of acetone-fixed HeLa cells labeling Histone H1.2 with ab181977 at 1/500 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/200 dilution (red). Dapi staining (blue).