Anti-Histone H1.3 (phospho T17) + Histone H1.4 (phospho T17) antibody [EPR18087]

• Supplier: Abcam
• Catalog: ab188294
• Prices: $99.00
• Sizes: 10 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR18087
• Applications: IHC-P ICC/IF ICC/IF WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Histone H1
• Description: Rabbit Monoclonal
• Gene: HIST1H1D
• Conjugate: Unconjugated

Product images

All lanes : Anti-Histone H1.3 (phospho T17) + Histone H1.4 (phospho T17) antibody [EPR18087] (ab188294) at 1/5000 dilutionLane 1 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysatesLane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) treated with Colcemid acid extraction lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-Histone H1.3 (phospho T17) + Histone H1.4 (phospho T17) antibody [EPR18087] (ab188294) at 1/5000 dilutionLane 1 : Untreated NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysatesLane 2 : NIH/3T3 (Mouse embyro fibroblast cells) treated with 1.5µg/ml Colcemid for 12 hours whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H1.3 (phospho T17) + Histone H1.4 (phospho T17) with ab188294 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.Nucleus staining on epithelial cells of Human colon is observed.Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Histone H1.3 (phospho T17) + Histone H1.4 (phospho T17) with ab188294 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.Nucleus staining on epithelial cells of mouse colon is observed.Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Histone H1.3 (phospho T17) + Histone H1.4 (phospho T17) with ab188294 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.Nucleus staining on epithelial cells of rat colon is observed.Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H1.3 (phospho T17) + Histone H1.4 (phospho T17) with ab188294 at 1/8000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).Confocal image showing nuclear staining on HeLa cell line.The nuclear counter stain is DAPI (blue).Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:-ve control 1: ab188294 at 1/8000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.