Anti-Histone H1.3 antibody

• Supplier: Abcam
• Catalog: ab24174
• Prices: $380.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P ICC/IF ICC/IF WB
• Species Reactivities: Human
• Antigen: Synthetic peptide: LAPTIPAPAEKTPVKKKAKKAGATAGK -C , corresponding to N terminal amino acids 7-33 of Human Histone H1
• Description: Rabbit Polyclonal
• Gene: HIST1H1D
• Conjugate: Unconjugated

Product images

Human 293T cells fixed in 4% paraformaldehyde were immunostained with ab24174 (1/200) for Histone H1.3 and detected using FITC labelled anti-rabbit (Green). Nuclear DNA is stained blue with DAPI.

IHC image of ab24174 staining in human cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24174, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

Histone H1 variants are differentially expressed and incorporated into chromatin - Histone H1 variants are differentially expressed and incorporated into chromatin

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Specialized compartments of cardiac nuclei exhibit distinct proteomic anatomy. - Specialized compartments of cardiac nuclei exhibit distinct proteomic anatomy.

Franklin S, Zhang MJ, Chen H, Paulsson AK, Mitchell-Jordan SA, Li Y, Ping P, Vondriska TM. Mol Cell Proteomics. 2011 Jan;10(1):M110.000703.

Linker histone H1 is present in centromeric chromatin of living human cells next - Linker histone H1 is present in centromeric chromatin of living human cells next

Orthaus S, Klement K, Happel N, Hoischen C, Diekmann S. Nucleic Acids Res. 2009 Jun;37(10):3391-406.

H1 histone subtype constitution and phosphorylation state of the ageing cell - H1 histone subtype constitution and phosphorylation state of the ageing cell

Happel N, Doenecke D, Sekeri-Pataryas KE, Sourlingas TG. Exp Gerontol. 2008 Mar;43(3):184-99.

Depletion of human histone H1 variants uncovers specific roles in gene expression - Depletion of human histone H1 variants uncovers specific roles in gene expression

Sancho M, Diani E, Beato M, Jordan A. PLoS Genet. 2008 Oct;4(10):e1000227.