Anti-Histone H1.5 antibody

• Supplier: Abcam
• Catalog: ab24175
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB IP
• Species Reactivities: Human
• Antigen: Synthetic peptide: AETATPAPVEKSPAKKKATKKAAGAGAAK -C , corresponding to N terminal amino acids 7-35 of Human Histone H1
• Description: Rabbit Polyclonal
• Gene: HIST1H1B
• Conjugate: Unconjugated

Product images

ICC/IF image of ab24175 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab24175 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Interactions of MCP1 with components of the replication machinery in mammalian - Interactions of MCP1 with components of the replication machinery in mammalian

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Histone H1 variants are differentially expressed and incorporated into chromatin - Histone H1 variants are differentially expressed and incorporated into chromatin

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Linker histone H1 is present in centromeric chromatin of living human cells next - Linker histone H1 is present in centromeric chromatin of living human cells next

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H1 histone subtype constitution and phosphorylation state of the ageing cell - H1 histone subtype constitution and phosphorylation state of the ageing cell

Happel N, Doenecke D, Sekeri-Pataryas KE, Sourlingas TG. Exp Gerontol. 2008 Mar;43(3):184-99.

Depletion of human histone H1 variants uncovers specific roles in gene expression - Depletion of human histone H1 variants uncovers specific roles in gene expression

Sancho M, Diani E, Beato M, Jordan A. PLoS Genet. 2008 Oct;4(10):e1000227.