Anti-Histone H2A (acetyl K9) antibody
| Name | Anti-Histone H2A (acetyl K9) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab47816 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Human, Mouse, Rat, Bovine |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H2A, acetylated at K9 |
| Blocking Peptide | Human Histone H2A (acetyl K9) peptide |
| Description | Rabbit Polyclonal |
| Gene | HIST1H2AE |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Histone H2A (acetyl K9) antibody (ab47816) at 1 µg/ml + HeLa whole cell lysate at 10 µgSecondaryDonkey Anti-Rabbit IgG H&L (HRP) (ab16284) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Performed under reducing conditions.
ICC/IF image of ab47816 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47816, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2, and MCF-7 cells at 5µg/ml, and in 100% Methanol fixed (5 min) HeLa, Hek293, HepG2, and MCF-7 cells at 5µg/ml.