Anti-Histone H2A.X (phospho S139) antibody [GT2311]

Name	Anti-Histone H2A.X (phospho S139) antibody [GT2311]
Supplier	Abcam
Catalog	ab184520
Prices	$384.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone	GT2311
Applications	ICC/IF ICC/IF IP WB
Species Reactivities	Mouse, Human, Rat, Bovine, Monkey
Antigen	The details of the immunogen for this antibody are not available
Description	Mouse Monoclonal
Gene	H2AFX
Conjugate	Unconjugated
Supplier Page	Shop

Product images

ab184520 detects H2A.X protein at nuclear by immunofluorescent analysis. Sample: 10uM Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min. Green: H2A.X protein stained with ab184520 diluted at 1:500. Blue: Hoechst 33342 staining. Bottom panels show merged images

ab184520 detects H2A.X protein at nuclear by confocal immunofluorescent analysis.Sample: 10nM Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min.Red: H2A.X protein stained with ab184520 diluted at 1:500.Green: alpha Tubulin antibody diluted at 1:1000.Blue: Hoechst 33342 staining. Bottom panels show merged images.

All lanes : Anti-Histone H2A.X (phospho S139) antibody [GT2311] (ab184520) at 1/1000 dilutionLane 1 : HCT116 whole cell lysate (untreated)Lane 2 : HCT116 whole cell lysate (30 µM cisplatin treatment for 24hr)Lysates/proteins at 30 µg per lane.

All lanes : Anti-Histone H2A.X (phospho S139) antibody [GT2311] (ab184520) at 1/1000 dilutionLane 1 : NIH-3T3 whole cell lysate(untreated)Lane 2 : NIH-3T3 whole cell lysate (30 µM cisplatin treatment for 24hr)Lysates/proteins at 30 µg per lane.

ab184520 immunoprecipitates histone H2A.X (phospho S139) protein in IP at 2 μg.1. 500 μg HCT116 with CPT 30 μM treatment 24 hr whole cell lysate/extract.2. 30 μg HCT116 whole with CPT 30 uM treatment cell lysate/extract .3. Control with 2 μg of preimmune mouse IgG C. The immunoprecipitated histone H2A.X (phospho S139) protein was detected by ab184520 diluted at 1:1000. 15% SDS-PAGE. Anti-mouse IgG was used as a secondary reagent.