Anti-Histone H2A-Bbd antibody
| Name | Anti-Histone H2A-Bbd antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab4175 |
| Prices | $370.00 |
| Sizes | 200 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide corresponding to Human Histone H2A-Bbd aa 50 to the C-terminus |
| Blocking Peptide | Human Histone H2A-Bbd (unmodified ) peptide |
| Description | Rabbit Polyclonal |
| Gene | H2AFB2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
H2A-Bbd (ab4175) CE= chicken erythrocyte (10 and 2 micrograms) , HE= HeLa cells (10 micrograms) and 2A*= recombinant H2ABbd (the numbers underneath the lanes are the number of nanograms loaded in each lane).The primary antibody dilution was 1:500 and the secondary was 1:3000.As the WB shows, we can not find any reaction with any of the different histone extracts that we have used from different sources (calf, human, chicken). However, the antibody is very specific against the recombinant form of the protein.
Mouse embryonic fibroblasts (MEFs) were fixed in 4%PFA, permeabilised in PBS 1%BSA 0.1%TX-100 and incubated with ab4175 (anti-H2A.Bbd) antibody o/n. The antibody appears to show nucleolar staining. The antibody gave the same staining pattern in different cell types. The dilution used was 3ug/ml.
Product References
Changes in the nuclear deposition of histone H2A variants during pre-implantation - Changes in the nuclear deposition of histone H2A variants during pre-implantation
Nashun B, Yukawa M, Liu H, Akiyama T, Aoki F. Development. 2010 Nov;137(22):3785-94.
Chromatin analysis of occluded genes. - Chromatin analysis of occluded genes.
Lee JH, Gaetz J, Bugarija B, Fernandes CJ, Snyder GE, Bush EC, Lahn BT. Hum Mol Genet. 2009 Jul 15;18(14):2567-74.