Anti-Histone H2B (phospho T129) antibody [EPR18095] - ChIP Grade

• Supplier: Abcam
• Catalog: ab188292
• Prices: $99.00
• Sizes: 10 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR18095
• Applications: WB ChIP
• Species Reactivities: Yeast
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Saccharomyces cerevisiae Histone H2B (yeast) aa 100 to the C-terminus (phospho T129)
• Description: Rabbit Monoclonal
• Gene: HTB2
• Conjugate: Unconjugated

Product images

All lanes : Anti-Histone H2B (phospho T129) antibody [EPR18095] - ChIP Grade (ab188292) at 1/100 dilutionLane 1 : Untreated Saccharomyces cerevisiae whole cell lysateLane 2 : Saccharomyces cerevisiae treated with 2 mg/ml Methyl methanesulfonate for 1 hour whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Chromatin was prepared from Saccharomyces cerevisiae cells according to the Abcam X-ChIP protocol. Saccharomyces cerevisiae cells were treated with MMS at 2mg/ml for 1 h. Treated and non-treated Saccharomyces cerevisiae cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab188292 (blue), and 20µl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).