Anti-Histone H3 (phospho S28) antibody
| Name | Anti-Histone H3 (phospho S28) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab5169 |
| Prices | $401.00 |
| Sizes | 200 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB Array ICC/IF ICC/IF ICC/IF |
| Species Reactivities | Human, Drosophila |
| Antigen | Synthetic peptide corresponding to Human Histone H3 aa 1-100 (phospho S28) conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Blocking Peptide | Human Histone H3 (phospho S28) peptide |
| Description | Rabbit Polyclonal |
| Gene | His3.3B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Histone H3 (phospho S28) antibody (ab5169) at 1 µg/ml + Hela Whole Cell Lysate - Colcemid Treated at 2.5 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All batches of ab5169 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - phospho S28 peptide (ab5499), indicating that this antibody specifically recognises the Histone H3 - phospho S28 modification.ab17163 - Histone H3 unmodifiedab5499 - Histone H3 - phospho S28ab11477 - Histone H3 - phospho S10
ab5169 staining Histone H3 (phospho S28) in human differentiated haematopoietic stem cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with permeabilization buffer. The sample was incubated with the primary antibody (1/400) for 12 hours at 4°C. An Allophycocyanin-conjugated goat polyclonal anti-rabbit IgG (1/1000) was used as the secondary antibody.Gating Strategy: Isotype negative control (white).See Abreview
Indian Muntjac (top panel) and HeLa cells (bottom panel) immunofluorescently labelled with ab5169 (green) at a working dilution of 1/5000. The DNA is counterstained with DAPI and is shown in blue in the top panel and red in the bottom panel. This antibody gives a characteristic staining pattern for Histone H3 (phospho S28) whereby the signal increases in intensity during late G2 and continues to increase until metaphase. Upon entry into anaphase the signal begins to decrease until reaching basal levels by early G1. 100x magnification.
All lanes : Anti-Histone H3 (phospho S28) antibody (ab5169) at 1/1000 dilutionLane 1 : Wild type 0-4 hour old fruit fly embryos.Lane 2 : 0-4 hour old fruit fly embryos expressing wee RNAiSecondaryDonkey anti-rabbit IgG, Horseradish Peroxidase-L at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
In situ peptide competition was perfomed on paraformaldehyde-fixed HeLa cells. Four 25µl aliquots were made, to which 7.5µg (1.5µl) of no peptide, H3 unmodified peptide (ab2623), H3 phospho S10 peptide (ab11477) or H3 phospho S28 peptide (ab5499) was added, mixed by vortexing and incubated for 1 hour at room temperature. Cells on glass coverslips were fixed with 4% paraformaldehyde (10min) and gently washed twice with PBS, then permeabilized with 0.5% Triton X-100 in PBS (10min) and gently washed three times with PBS. The cells were immunofluorescently labeled with either the peptide-competed antibody or the control antibody (i.e. no peptide) for 30min at room temperature, washed briefly with PBS containing 0.1% Triton X-100 (1 min) and twice with PBS. The cells were then incubated with an appropriate dilution of a secondary antibody at room temperature for 30min, rinsed as above and mounted using a 90% glycerol in PBS mount
Product References
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Phosphorylation of histone H3 Thr-45 is linked to apoptosis. - Phosphorylation of histone H3 Thr-45 is linked to apoptosis.
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A RasGAP SH3 peptide aptamer inhibits RasGAP-Aurora interaction and induces - A RasGAP SH3 peptide aptamer inhibits RasGAP-Aurora interaction and induces
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