Anti-Histone H3 (phospho S28) antibody [HTA28]

• Supplier: Abcam
• Catalog: ab10543
• Prices: $401.00
• Sizes: 100 µl
• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone: HTA28
• Applications: IHC-F ICC/IF ICC/IF WB ICC/IF FC
• Species Reactivities: Mouse, Chicken, Hamster, Bovine, Human, Drosophila, Marmoset
• Antigen: Synthetic peptide corresponding to Human Histone H3 aa 23-35 (phospho S28) conjugated to Keyhole Limpet Haemocyanin (KLH)
• Description: Rat Monoclonal
• Gene: H3F3A
• Conjugate: Unconjugated

Product images

All lanes : Anti-Histone H3 (phospho S28) antibody [HTA28] (ab10543) at 1 µg/mlLane 1 : HeLa Histone Preparation Nuclear Lysate - Colcemid-treatedLane 2 : HeLa Histone Preparation Nuclear Lysate - Colcemid-treated with Human Histone H3 (unmodified ) peptide (ab2623) at 0.5 µg/mlLane 3 : HeLa Histone Preparation Nuclear Lysate - Colcemid-treated with Human Histone H3 (phospho S28) peptide (ab5499) at 0.5 µg/mlLane 4 : HeLa Histone Preparation Nuclear Lysate - Colcemid-treated with Human Histone H3 (phospho S10) peptide (ab11477) at 0.5 µg/mlLysates/proteins at 2.5 µg per lane.SecondaryPeroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 17 kDa (why is the actual band size different from the predicted?)Exposure time : 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto

ab10543 staining HeLa cells by ICC/IF. The cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X100 in PBS. The cells were then stained with ab10543 at 1/1000 in PBS for 1h at 22°C. A goat anti-rat Alexa Fluro 488 (ab150157) at 1/200 was used as the secondary antibody. Nuclei are stained in red with DAPI. The antibody produces the expected mitotic-associated staining pattern and is extremely strong. MeOH fixed samples were also evaluated and produced a similar, strong staining pattern in mitotic cells.

ab10543 staining Histone H3 (phospho S28) in mouse embryonic brain tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocking with 10% serum for 1 hour at 250C was performed. The sample was incubated with primary antibody (1/300)  at 40C for 16 hours. An Alexa Fluor®488-conjugated Goat polyclonal to rat IgG was used as secondary antibody at 1/500 dilution.See Abreview

ab10543 staining Histone H3 (phospho S28) in murine embryonic fibroblast cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde and permeabilised in -20°C ethanol. Samples were then incubated with primary antibody at a 1/200 dilution for 1 hour in 0.1%Saponin/ 2.5% BSA/ PBS. The secondary antibody used was a goat anti-rat IgG (H+L) conjugated to DyLight® 649 (red) used at a 1/300 dilution. Gamma tubulin (blue), Ki67 (green) using ab15580.

Product References

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