Western blot analysis of extracts from various cell lines and tissues using BMAL1 (D2L7G) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human BMAL1 protein (hBMAL1-Myc; +), using BMAL1 (D2L7G) Rabbit mAb.
Immunoprecipitation of BMAL1 from SH-SY5Y cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or BMAL1 (D2L7G) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using BMAL1 (D2L7G) Rabbit mAb.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 3T3 cells treated with dexamethasone (1 μM) for 2 hours, and either 10 μl of BMAL1 (D2L7G) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using mouse DBP intron 2 primers, SimpleChIP ® Mouse PER1 Intron 1 Primers #13902, and SimpleChIP ® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.