All lanes : Anti-Histone H3 antibody (ab134198) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-Histone H3 antibody (ab134198) at 1 µg/mlLane 1 : Histone H2B Recombinant ProteinLane 2 : Histone H3.1 Recombinant ProteinLysates/proteins at 0.1 µg per lane.SecondaryGoat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-Histone H3 antibody (ab134198) at 1 µg/mlLane 1 : Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) at 20 µgLane 2 : S.cerevisiae (Y190) Whole Cell Lysate at 10 µgLane 3 : S.pombe Whole Cell Lysate at 20 µgSecondaryGoat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilutionPerformed under reducing conditions.