Anti-Histone H3 antibody - Nuclear Loading Control (HRP)

• Supplier: Abcam
• Catalog: ab21054
• Prices: $382.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB IHC-P
• Species Reactivities: Mouse, Human, Yeast, Drosophila
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Histone H3
• Blocking Peptide: Human Histone H3 peptide
• Description: Rabbit Polyclonal
• Gene: His3.3B
• Conjugate: HRP

Product images

IHC image of Histone H3 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21054, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

All lanes : Anti-Histone H3 antibody - Nuclear Loading Control (HRP) (ab21054) at 1/5000 dilutionLane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179) at 10 µgLane 2 : Yeast S.cerevisiae Whole Cell Lysate at 10 µgLane 3 : Drosophila Embryo Nuclear Extract at 20 µgPerformed under reducing conditions.

Product References

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Hiragami-Hamada K, Shinmyozu K, Hamada D, Tatsu Y, Uegaki K, Fujiwara S, Nakayama J. Mol Cell Biol. 2011 Mar;31(6):1186-200.

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Vizoso Vazquez A, Blanco M, Zaborowska J, Soengas P, Gonzalez-Siso MI, Becerra M, Rodriguez-Belmonte E, Cerdan ME. Eukaryot Cell. 2011 Oct;10(10):1331-9.

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Binda O, LeRoy G, Bua DJ, Garcia BA, Gozani O, Richard S. Epigenetics. 2010 Nov-Dec;5(8):767-75.

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Wei W, Chua MS, Grepper S, So SK. Mol Cancer. 2009 Sep 24;8:76.