Anti-Histone H4 (acetyl K8) antibody [EP1002Y] - ChIP Grade

• Supplier: Abcam
• Catalog: ab45166
• Prices: $391.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1002Y
• Applications: WB IHC-P ICC/IF ICC/IF IP ChIP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Histone H4 aa 1-100 (N terminal) (acetyl K8)
• Description: Rabbit Monoclonal
• Gene: HIST4H4
• Conjugate: Unconjugated

Product images

IHC image of ab45166 staining Histone H4 (acetyl K8) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45166, 1/200, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

All lanes : Anti-Histone H4 (acetyl K8) antibody [EP1002Y] - ChIP Grade (ab45166) at 1/10000 dilutionLane 1 : C6 cell lysateLane 2 : C6 cell + TSA lysate

Immunofluorescent staining of Hela cells using ab45166.

Ab45166 staining human Histone H4 in human cervical carcinoma by immunohistochemistry using paraffin embedded tissue.Ab45166 staining human Histone H4 in human cervical carcinoma by immunohistochemistry using paraffin embedded tissue.

Product References

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