Anti-Histone H4 (acetyl K8) antibody - ChIP Grade
| Name | Anti-Histone H4 (acetyl K8) antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab15823 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P Electron microscopy ChIP WB |
| Species Reactivities | Mouse, Chicken, Bovine, Human, Yeast, Drosophila, S. pombe |
| Antigen | Synthetic peptide corresponding to Human Histone H4 aa 1-100 (internal sequence) (acetyl K8) conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Description | Rabbit Polyclonal |
| Gene | His4:CG31611 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Histone H4 (acetyl K8) antibody - ChIP Grade (ab15823) at 1 µg/mlLane 1 : Fruit fly (Drosophila melanogaster) embryo tissue lysate at 5 µgLane 2 : Fruit fly (Drosophila melanogaster) embryo tissue lysate at 2.5 µgSecondaryHRP-conjugated Goat anti-rabbit IgG polyclonal at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC image of Histone H4 (acetyl K8) staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab15823, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab15823 (blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
All lanes : Anti-Histone H4 (acetyl K8) antibody - ChIP Grade (ab15823) at 1 µg/mlLane 1 : Sodium butyrate treated hela histone Lane 2 : Untreated hela histone Lane 3 : Sodium butyrate treated hela histone with Human Histone H4 (acetyl K8) peptide (ab15824) at 1 µg/mlLane 4 : Untreated hela histone with Human Histone H4 (acetyl K8) peptide (ab15824) at 1 µg/mlLane 5 : Sodium butyrate treated hela histone with Human Histone H4 peptide (ab15825) at 1 µg/mlLane 6 : Untreated hela histone with Human Histone H4 peptide (ab15825) at 1 µg/mlLane 7 : Sodium butyrate treated hela histone with Histone H4 peptide - acetyl K5 (ab15596) at 1 µg/mlLane 8 : Untreated hela histone with Histone H4 peptide - acetyl K5 (ab15596) at 1 µg/mlLane 9 : Sodium butyrate treated hela histone with Human Histone H4 (acetyl K12) peptide (ab15662) at 1 µg/mlLane 10 : Untreated hela histone with Human Histone H4 (acetyl K12) peptide (ab15662) at 1 µg/mlLane 11 : Sodium butyrate treated hela histone with Histone H2A p
ICC/IF image of ab15823 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab15823, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
A: Overall view of a murine rod photoreceptor nucleus immunogold labelled with acetylated H4K8-specific antibodies.B: Schematic representation of the H4K8ac-specific labelling. The bar represents 1µm.
All lanes : Anti-Histone H4 (acetyl K8) antibody - ChIP Grade (ab15823) at 1 µg/mlLane 1 : Calf Thymus Histone Preparation Nuclear Lysate (ab121)Lane 2 : Calf Thymus Histone Preparation Nuclear Lysate (ab121) with Human Histone H4 peptide (ab15825) at 0.5 µg/mlLane 3 : Calf Thymus Histone Preparation Nuclear Lysate (ab121) with Human Histone H4 (acetyl K8) peptide (ab15824) at 0.5 µg/mlLane 4 : Calf Thymus Histone Preparation Nuclear Lysate (ab121) with Histone H4 peptide - acetyl K5 at 0.5 µg/mlLane 5 : Calf Thymus Histone Preparation Nuclear Lysate (ab121) with Human Histone H4 (acetyl K12) peptide (ab15662) at 0.5 µg/mlLane 6 : Calf Thymus Histone Preparation Nuclear Lysate (ab121) with Human Histone H2A (acetyl K9) peptide (ab54016) at 0.5 µg/mlLysates/proteins at 0.5 µg/ml per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
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