Anti-Histone H4 (acetyl K91) antibody - ChIP Grade
| Name | Anti-Histone H4 (acetyl K91) antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab4627 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ChIPseq ICC/IF ICC/IF IP ChIP |
| Species Reactivities | Mouse, Bovine, Yeast, Rat, Chicken, Human, Pig, Xenopus, C. elegans, Drosophila, Duck |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Histone H4, acetylated at K91 |
| Description | Rabbit Polyclonal |
| Gene | His4:CG31611 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab4627 staining Histone H4 (acetyl K91) in mouse C2C12 muscle fibroblast cultured cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 20 minutes at 23°C. Samples were incubated with primary antibody (1/1000 in 3% BSA + 0.1% Triton X-100 in PBS) for 12 hours at 4°C. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/2000) was used as the secondary antibody.See Abreview
IHC image of ab4627 staining Histone H2A.X in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4627, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab4627 (blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
Product References
Histone acetyltransferase 1 promotes homologous recombination in DNA repair by - Histone acetyltransferase 1 promotes homologous recombination in DNA repair by
Yang X, Li L, Liang J, Shi L, Yang J, Yi X, Zhang D, Han X, Yu N, Shang Y. J Biol Chem. 2013 Jun 21;288(25):18271-82.
Single nucleosome ChIPs identify an extensive switch of acetyl marks on cell - Single nucleosome ChIPs identify an extensive switch of acetyl marks on cell
Gatta R, Mantovani R. Cell Cycle. 2010 Jun 1;9(11):2149-59. Epub 2010 Jun 1.
Screen for DNA-damage-responsive histone modifications identifies H3K9Ac and - Screen for DNA-damage-responsive histone modifications identifies H3K9Ac and
Tjeertes JV, Miller KM, Jackson SP. EMBO J. 2009 Jul 8;28(13):1878-89.
BBAP monoubiquitylates histone H4 at lysine 91 and selectively modulates the DNA - BBAP monoubiquitylates histone H4 at lysine 91 and selectively modulates the DNA
Yan Q, Dutt S, Xu R, Graves K, Juszczynski P, Manis JP, Shipp MA. Mol Cell. 2009 Oct 9;36(1):110-20.
Combinatorial patterns of histone acetylations and methylations in the human - Combinatorial patterns of histone acetylations and methylations in the human
Wang Z, Zang C, Rosenfeld JA, Schones DE, Barski A, Cuddapah S, Cui K, Roh TY, Peng W, Zhang MQ, Zhao K. Nat Genet. 2008 Jul;40(7):897-903.
Site specificity of yeast histone acetyltransferase B complex in vivo. - Site specificity of yeast histone acetyltransferase B complex in vivo.
Poveda A, Sendra R. FEBS J. 2008 May;275(9):2122-36.
Analysis of Myc-induced histone modifications on target chromatin. - Analysis of Myc-induced histone modifications on target chromatin.
Martinato F, Cesaroni M, Amati B, Guccione E. PLoS One. 2008;3(11):e3650.
Myc-binding-site recognition in the human genome is determined by chromatin - Myc-binding-site recognition in the human genome is determined by chromatin
Guccione E, Martinato F, Finocchiaro G, Luzi L, Tizzoni L, Dall' Olio V, Zardo G, Nervi C, Bernard L, Amati B. Nat Cell Biol. 2006 Jul;8(7):764-70. Epub 2006 Jun 11.