This antibody gave a positive result in ELISA against the immunizing peptide ab26421 (blue line). For the following peptides it gave following results:Non-modified equivalent peptide ab34435 (purple line) - very weak result, Histone H3 Hydroxy P16+P30 peptide ab17162 (yellow line) - negative result.This indicates that it is specific for the modified peptide.
ICC/IF image of ab26104 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab26104, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HEK 293, HepG2 and MCF7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.