Anti-Histone H4 (mono methyl K16) antibody [EPR17910]

• Supplier: Abcam
• Catalog: ab177849
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR17910
• Applications: WB ICC/IF ICC/IF
• Species Reactivities: Mouse, Human, Broad species
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Histone H4 aa 1-100 (mono methyl K16) (Cysteine residue)
• Description: Rabbit Monoclonal
• Gene: HIST4H4
• Conjugate: Unconjugated

Product images

All lanes : Anti-Histone H4 (mono methyl K16) antibody [EPR17910] (ab177849) at 1/2000 dilutionLane 1 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysateLane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H4 (mono methyl K16) with ab177849 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:-ve control 1: ab177849 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.