Anti-Histone H4 (mono methyl R3) antibody

• Supplier: Abcam
• Catalog: ab17339
• Prices: $380.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB DB ELISA IHC-P ICC/IF ICC/IF Array
• Species Reactivities: Human, Mouse, Rat, Bovine, Pig
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H4, mono methylated at R3
• Blocking Peptide: Human Histone H4 (mono methyl R3) peptide
• Description: Rabbit Polyclonal
• Gene: HIST4H4
• Conjugate: Unconjugated

Product images

ab17339 staining Histone H4 (mono methyl R3) in human differentiated haematopoietic stem cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with permeabilization buffer. The sample was incubated with the primary antibody (1/800) for 12 hours at 4°C. An Allophycocyanin-conjugated goat polyclonal anti-rabbit IgG (1/1000) was used as the secondary antibody.Gating Strategy: Isotype negative control (white).See Abreview

Anti-Histone H4 (mono methyl R3) antibody (ab17339) at 1 µg/ml + HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

All batches of ab17339 are tested in Peptide Array against peptides to different Histone H4 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H4 - mono methyl R3 peptide (ab17770), indicating that this antibody specifically recognises the Histone H4 - mono methyl R3 modification.ab17770 - Histone H4 - mono methyl R3ab15821 - Histone H4 - unmodified

Image courtesy of Human Protein Atlas ab17339 staining histone H4 mono methyl R3 in human testis, showing a distinct and strong staining pattern in ductus seminiferus an leydig cells. Paraffin embedded human skin tissue was incubated with ab17339 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab17339 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org.

ICC/IF image of ab17339 stained human HepG2 cells. The cells were PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab17339, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

Product References

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Global histone modification patterns as prognostic markers to classify glioma - Global histone modification patterns as prognostic markers to classify glioma

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JMJD6 is a histone arginine demethylase. - JMJD6 is a histone arginine demethylase.

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